|
| Status |
Public on Oct 02, 2018 |
| Title |
9-100-SpinalCord_S9 |
| Sample type |
SRA |
| |
|
| Source name |
Spinal cord_100
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
severity: 100
tissue: Spinal cord
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions, eluting in 30 μL of water.
500 ng RNA was used for library preparation with Illumina’s TruSeq Stranded mRNA kit (Illumina, San Diego, CA).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
S9.100.SpinalCord_S9_R1_001
|
| Data processing |
Quality control: FastQC
Pseudoalignment: Salmon (version 0.8.2)
Genome_build: Rnor_6.0.89
Supplementary_files_format_and_content: pseudocounts
|
| |
|
| Submission date |
May 30, 2018 |
| Last update date |
Oct 02, 2018 |
| Contact name |
Jordan W Squair |
| Organization name |
University of British Columbia
|
| Street address |
818 West 10th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z1M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL25029 |
| Series (1) |
| GSE115067 |
Integrated systems analysis reveals conserved gene networks underlying response to spinal cord injury |
|
| Relations |
| BioSample |
SAMN09280245 |
| SRA |
SRX4139410 |
