|
| Status |
Public on Sep 03, 2008 |
| Title |
Cont subject 04 |
| Sample type |
RNA |
| |
|
| Source name |
myotubes from control subject
|
| Organism |
Homo sapiens |
| Characteristics |
The control subjects had normal glucose tolerance and no family history of diabetes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from muscle cell cultures using RNeasy (Qiagen)
|
| Label |
biotin
|
| Label protocol |
In vitro transcription was performed on 1 µg of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics, USA)
|
| |
|
| Hybridization protocol |
10 µg of fragmented cRNA were hybridised to a U95Av2 GeneChip (Affymetrix) using their standard procedure (45°C, 16-h). Washing and staining was performed in a Fluidics Station 400 (Affymetrix) using the protocol EukGE-WS2v4.
|
| Scan protocol |
Scanned in an Affymetrix GeneChip 2500 scanner.
|
| Description |
control subject 04
|
| Data processing |
Microarray normalisation and calculation of expression measures were performed using Robust Multiarray Average, implemented in the statistical package R provided by Bioconductor (Seattle, WA, USA). The expression values were log 2 transformed to obtain a normal distribution across arrays and samples. Before analysing the expression data we removed Affymetrix control probes, resulting in a total of 12,558 probes.
|
| |
|
| Submission date |
Sep 02, 2008 |
| Last update date |
Mar 16, 2009 |
| Contact name |
Casper Frederiksen |
| Organization name |
Odense University Hospital
|
| Street address |
Kloevervaenget 6
|
| City |
Odense |
| ZIP/Postal code |
DK-5000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE12643 |
Transcription profiling of myotubes from patients with type 2 diabetes |
|
