|
| Status |
Public on Jan 12, 2009 |
| Title |
breast cancer cell line_MDA-MB-468 |
| Sample type |
RNA |
| |
|
| Source name |
Breast cancer
|
| Organism |
Homo sapiens |
| Characteristics |
gene_expression_subtype: Basal
Her2_status: Nonamplified
|
| Treatment protocol |
Cells untreated
|
| Growth protocol |
Cell maintained in RPMI1640 or DMEM supplemented with 10% fetal bovine serum, non-essential amino acids and 2mmol/L L-glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
breast cancer cell line_MDA-MB-468
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Sep 15, 2008 |
| Last update date |
Feb 29, 2012 |
| Contact name |
Mark Lackner |
| E-mail(s) |
[email protected]
|
| Organization name |
Genentech
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE12777 |
Gene expression profiling of 51 human breast cancer cell lines |
| GSE12790 |
Gene expression profiling of human breast cancers and cancer cell lines |
|
| Relations |
| Reanalysis of |
GSM276009 |
