|
| Status |
Public on Sep 23, 2008 |
| Title |
gun5_norflurazon_1 |
| Sample type |
RNA |
| |
|
| Source name |
gun5 NF treated
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
whole seedling
|
| Treatment protocol |
Sedds were grown on plates with or without 5µM Norflurazon
|
| Growth protocol |
Seeds were surface sterilized and plated on 1/2 LS plates supplemented with 1% sucrose and grown at 21o C under continous light (100µmol*m-2*sec-1).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy (Qiagen; Hilden, Geramny) according to manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Affymetrix
|
| |
|
| Hybridization protocol |
Affymetrix
|
| Scan protocol |
Affymetrix
|
| Description |
5 day old light grown
|
| Data processing |
Data manipulations were performed in R (http://www.r-project.org/). Standard Affymetrix quality-control procedures were carried out using the BioConductor packages simpleaffy and affyPLM. Expression was normalized and estimated using the gcRMA package of BioConductor. The quality of data from replicate samples was checked using least square regression (R2) and scatter plots for each condition studied. The normalized data were filtered, using two criteria: (1) detectable expression under at least one condition as defined by a MAS5 (Affymetrix) and normalized expression larger than 60 units and variable expression across conditions (p value < 0.01 from ANOVA modeling)
|
| |
|
| Submission date |
Sep 22, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Joanne Chory |
| E-mail(s) |
[email protected]
|
| Phone |
858-453-4100
|
| URL |
http://cactus.salk.edu
|
| Organization name |
The Salk Institute for Biological Studies
|
| Department |
Plant Biology Laboratory
|
| Street address |
10010 N. Torrey Pines Road.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE12887 |
Differential response of gun mutants to norflurazon |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
