|
| Status |
Public on Jan 27, 2011 |
| Title |
colonic epithelium_E-infection_day1_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
colonic epithelium
|
| Organism |
Mus musculus |
| Characteristics |
Source Type isolated cell
Organism Part large intestine
Cell Type epithelial cell
Cell Purification n/a
Strain[background] BALB/c
Developmental Stage adult
Age 16 weeks
Sex male
Microbial Condition gnotobiote
Genetic Modification n/a
Disease State healthy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
using commercial kit
|
| Label |
biotin
|
| Label protocol |
One-Cycle Target Labeling and Control Reagents
|
| |
|
| Hybridization protocol |
Affymetrix standard procedures
|
| Scan protocol |
using GeneChip Scanner 3000
|
| Description |
Isolation Protocol: Small pieces of colon were dissected from mouse large intestine and soaked in Hank's balanced salt solution (HBSS) containing 30 mM EDTA. After incubation on ice for 20 min, IECs were isolated by manipulation with a fine needle under stereomicroscopic monitoring.
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Oct 01, 2008 |
| Last update date |
Jan 27, 2011 |
| Contact name |
Shinji Fukuda |
| E-mail(s) |
[email protected]
|
| Organization name |
Keio University
|
| Department |
Institute for Advanced Biosciences
|
| Lab |
Microbiome Research Group
|
| Street address |
246-2 Mizukami, Kakuganji
|
| City |
Tsuruoka |
| State/province |
Yamagata |
| ZIP/Postal code |
997-0052 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE12998 |
Comparative transcriptomic analysis of BA- or BL- associated murine colonic epithelium after O157 infection |
| GSE13061 |
Comparative transcriptomic analysis of BA- or BL- |
|
