|
| Status |
Public on Aug 30, 2018 |
| Title |
MM134_LTED_B_1 |
| Sample type |
SRA |
| |
|
| Source name |
MM134
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MM134
cell type: long-term estrogen-deprived (LTED)
cell line type: Invasive lobular breast carcinoma
|
| Treatment protocol |
Parental and LTED MM134 and SUM44 cells were seeded in triplicates in 6-well plates. Parental cells were hormone deprived for three days before cell collection. For hormone deprivation, cells were washed twice daily for 3 days. For each wash, cells were rinsed twice with serum-free IMEM and then cultured in IMEM + 10% CSS. A minimum 1-hour interval was kept between two washes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Illustra RNAspin Mini Kit
TruSeq Stranded Total RNA Library Prep Kit was used for library preparation according to Illumina standard protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Raw sequence data were mapped to hg38 genome (ensembl release version 82) and gene counts were quantified with Salmon (version 0.6.0)
Genome_build: hg38
|
| |
|
| Submission date |
Jul 06, 2018 |
| Last update date |
Aug 30, 2018 |
| Contact name |
Kevin Levine |
| E-mail(s) |
[email protected]
|
| Phone |
4126417736
|
| Organization name |
University of Pittsburgh
|
| Street address |
204 Craft Ave
|
| City |
Pittsburgh |
| State/province |
Pennsylvania |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE116744 |
RNA-Seq analysis of long-term estrogen-deprived (LTED) MDA-MB-134VI (MM134) and SUM44PE (SUM44) ILC cell lines |
|
| Relations |
| BioSample |
SAMN09623857 |
| SRA |
SRX4349362 |
