 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 28, 2019 |
| Title |
U1_HACA_siCTRL_02 |
| Sample type |
SRA |
| |
|
| Source name |
cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa with transfected U1 chimera
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of RNA
Kapa hyper-prep (KK8502) of RACE amplicons using illumina TruSeq (RS-122-2001, RS-122-2002) barcodes. Briefly, RACE amplicons of different RNAs from the same sample were pooled into one index, and libraries were prepared according to manufacturer protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
RACE |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
Paired-end joining using fastq-join (Galaxy Version 1.1.2-806.1)
Isolated 3' sequence ends with custom python scripts ()
Binned 3' ends into categories with custom python scripts ()
Supplementary_files_format_and_content: csv files were generated using custom python scripts ()
|
| |
|
| Submission date |
Jul 12, 2018 |
| Last update date |
Mar 28, 2019 |
| Contact name |
Caitlin M Roake |
| E-mail(s) |
[email protected]
|
| Phone |
6504004908
|
| Organization name |
Stanford
|
| Lab |
Artandi
|
| Street address |
CCSR 1150, 269 Campus Drive
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL15520 |
| Series (1) |
| GSE117054 |
Maturation of human telomerase RNA component from an extended precursor |
|
| Relations |
| BioSample |
SAMN09652347 |
| SRA |
SRX4386983 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
