|
| Status |
Public on Mar 05, 2010 |
| Title |
Lcl-control-3 |
| Sample type |
RNA |
| |
|
| Source name |
Control (46,XY)
|
| Organism |
Homo sapiens |
| Characteristics |
Cell type: Lymphoblastoid cell lines
Gender: Male
Age: 6
|
| Growth protocol |
Cells were cultured in RPMI-1640 Medium with GlutaMAX (Gibco, Invitrogen), supplemented with 10% (v/v) Fetal calf serum, and 1% (v/v) penicillin and streptomycin at 37oC in 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol reagent (Invitrogen, Carlsbad, NM, USA), cleaned on RNeasy columns (Qiagen) according to the manufacturers' instructions
|
| Label |
biotin
|
| Label protocol |
200ng of total RNA were amplified and labeled using the Message Amp II-biotin Enhanced reagents (Ambion; catalog #AM1791)
|
| |
|
| Hybridization protocol |
Affymetrix HG-U133 Plus 2.0 arrays were hybridized with 11 microgramme of labeled, amplified cRNA , washed, stained and scanned according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol EukGeWS2v5_450)
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 7G
|
| Description |
Comparison of the transcriptomes of cell lines from control and t(11;22)(q23;q11) individuals.
|
| Data processing |
Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
|
| |
|
| Submission date |
Oct 08, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Frédéric Schütz |
| E-mail(s) |
[email protected]
|
| Organization name |
Swiss Institute of Bioinformatics
|
| Street address |
Batiment Amphipole
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE13122 |
The Effect of Translocation-Induced Nuclear Re-organization on Gene Expression |
|
| Relations |
| Reanalyzed by |
GSE119087 |
