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Sample GSM328668 Query DataSets for GSM328668
Status Public on Mar 05, 2010
Title Lcl-control-8
Sample type RNA
 
Source name Control (46,XX)
Organism Homo sapiens
Characteristics Cell type: Lymphoblastoid cell lines
Gender: Female
Age: 26
Growth protocol Cells were cultured in RPMI-1640 Medium with GlutaMAX (Gibco, Invitrogen), supplemented with 10% (v/v) Fetal calf serum, and 1% (v/v) penicillin and streptomycin at 37oC in 5% CO2
Extracted molecule total RNA
Extraction protocol TRIzol reagent (Invitrogen, Carlsbad, NM, USA), cleaned on RNeasy columns (Qiagen) according to the manufacturers' instructions
Label biotin
Label protocol 200ng of total RNA were amplified and labeled using the Message Amp II-biotin Enhanced reagents (Ambion; catalog #AM1791)
 
Hybridization protocol Affymetrix HG-U133 Plus 2.0 arrays were hybridized with 11 microgramme of labeled, amplified cRNA , washed, stained and scanned according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol EukGeWS2v5_450)
Scan protocol Scanning was done on an Affymetrix GeneChip Scanner 7G
Description Comparison of the transcriptomes of cell lines from control and t(11;22)(q23;q11) individuals.
Data processing Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
 
Submission date Oct 08, 2008
Last update date Aug 28, 2018
Contact name Frédéric Schütz
E-mail(s) [email protected]
Organization name Swiss Institute of Bioinformatics
Street address Batiment Amphipole
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platform ID GPL570
Series (1)
GSE13122 The Effect of Translocation-Induced Nuclear Re-organization on Gene Expression
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Log2 normalized expression signal

Data table
ID_REF VALUE
1007_s_at 7.087571256
1053_at 8.268210654
117_at 5.578368208
121_at 8.076723973
1255_g_at 2.919532658
1294_at 7.35550622
1316_at 5.575864185
1320_at 4.283778496
1405_i_at 7.945519698
1431_at 3.406641164
1438_at 4.041082017
1487_at 7.107393742
1494_f_at 4.620793679
1552256_a_at 9.044049347
1552257_a_at 8.071887259
1552258_at 4.591153729
1552261_at 4.433575585
1552263_at 8.444327236
1552264_a_at 8.136580873
1552266_at 2.359091996

Total number of rows: 54675

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM328668.CEL.gz 7.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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