Sparged, fermentor clutures of the <i>msn2/4</i> mutant strain were aerobically grown on galactose medium (SSG-TEA). At time zero (generation 0) the saprge gas was switched from air to O<sub>2</sub>-free N<sub>2</sub> and samples were harvested after 0 (aerobic control), 0.04, 0.08, 0.19, and 2 generations of anaerobic growth. 30 µg of total RNA was reverse transcribed (Cy3) and hybridized against a reference (Cy5), which consisted of a pool of equal masses of RNA from each time point sampled. The data are background-corrected and normalized.
