|
| Status |
Public on Dec 01, 2008 |
| Title |
3H LPS, patient 89, nonresponder |
| Sample type |
RNA |
| |
|
| Source name |
arterial blood mononuclear cells activated by LPS, nonresponder
|
| Organism |
Homo sapiens |
| Characteristics |
race: Caucasian
tissue: blood
cell type: mononuclear cells
treatment: LPS
disease: chronic coronary occlusion
collateral flow response: insufficient
|
| Treatment protocol |
Subsequently, monocytes were negatively isolated using immunomagnetic beads for stimulation with 10ng/ml LPS for 3h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was extracted from 10 and 10 patients from the extreme ends of the spectrum (very high vs. very low CFI), by using Trizol (Invitrogen) with an RNEasy Micro column cleanup (Qiagen), who were matched for age, sex, medication and other factors that influence collateral artery growth.
|
| Label |
Biotin
|
| Label protocol |
Default in vitro transcription to produced Biotinylated antisense aRNA by using the Illumina Totalprep RNA amplification kit (#IL1791, Ambion).
|
| |
|
| Hybridization protocol |
750ng of biotinylated cRNA was hybridized onto the HumanRef-8 Expression BeadChips according to the manufacturer's specifications.
|
| Scan protocol |
According to the manufacturer, performed by ServiceXS (http://www.servicexs.com).
|
| Description |
patient: Caucasian patients were included who underwent percutaneous coronary intervention (PCI) of a total coronary occlusion. Exclusion criteria were previous myocardial infarction, cardiac surgery, depressed left ventricular function, diabetes mellitus, neoplastic or inflammatory disease. Invasive coronary collateral flow index (CFI) measurements were then performed. Patients were dichotomized as either sufficient (CFI>0.37) or insufficient (CFI≤0.37) collateral responders (0.37 was the median CFI of this patient group).
tissue: mononuclear cells were collected from 50 ml of arterial blood using Ficoll separation
|
| Data processing |
Gene expression analysis was performed by using Illumina's Beadstudio v3 software, followed by a quantile inter-array normalization (R, Bioconductor).
|
| |
|
| Submission date |
Oct 21, 2008 |
| Last update date |
Jul 28, 2010 |
| Contact name |
Oscar Leonard Volger |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
Academic Medical Center
|
| Department |
Cardiology
|
| Street address |
Meibergdreef 15
|
| City |
Amsterdam |
| State/province |
Noor Holland |
| ZIP/Postal code |
1105 AZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL5104 |
| Series (1) |
| GSE13290 |
Circulating Cells in Coronary Collateral Artery Growth II |
|
