Treated daily (9:00 am) with MPTP hydrochloride (0.2 mg/kg, i.v., Sigma, St. Louis, MO) dissolved in saline. Sacrificed in ketamine anesthesia by sodium pentobarbital overdose
Growth protocol
Animals were housed in individual primate cages under controlled conditions of humidity (50 F 5%), temperature (24 F 18C) and light (12h light/12h dark cycles, time lights on 8:00 am), food and water were available ad libitum and animal care was supervised by veterinarians skilled in the healthcare and maintenance of nonhuman primates. Experiments were carried out in accordance with European Communities Council Directive of 24 November 1986 (86/609/EEC) for care of laboratory animals.
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA from the prefrontal cortex sample was performed according to the manufacturer's instructions.
Label
biotin
Label protocol
According to the standard protocol outlined in the Affymetrix GeneChip Expression Analysis Technical Manual
Hybridization protocol
According to the manufacturer's instructions.
Scan protocol
At 3-Am resolution using the GeneChip System confocal scanner (Hewlett-Packard, Santa Clara, CA) with a 488-nm emission and detection at 70 nm, controlled by Affymetrix Microarray Suite 5.0 (Affymetrix).
Description
Putamen of MPTP treated macaque, day 6, replicate 5.
