|
| Status |
Public on Oct 06, 2020 |
| Title |
RANK-/- vs RANK+/+ MMTV-PYMT tumor cells Array 1B |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
RANK-/-
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Flow cytometry-sorted CD45- MMTV-PYMT tumor cells
strain: C57BL/6
genotype: RANK-/-
|
| Growth protocol |
One million MMTV-PyMT late carcinoma tumor cells (either RANK+/+ or RANK-/-) were orthotopically transplanted into syngeneic C57BL/6 mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Grown tumors were enzymatically disaggregated and live CD45- cells were sorted using MoFlo Astrios.
|
| Label |
Cy5
|
| Label protocol |
Two-color microarray-based LowInput QuickAmp Labeling (Agilent)
|
| |
|
| Channel 2 |
| Source name |
RANK+/+
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: RANK+/+
tissue: Flow cytometry-sorted CD45- MMTV-PYMT tumor cells
|
| Growth protocol |
One million MMTV-PyMT late carcinoma tumor cells (either RANK+/+ or RANK-/-) were orthotopically transplanted into syngeneic C57BL/6 mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Grown tumors were enzymatically disaggregated and live CD45- cells were sorted using MoFlo Astrios.
|
| Label |
Cy3
|
| Label protocol |
Two-color microarray-based LowInput QuickAmp Labeling (Agilent)
|
| |
|
| |
| Hybridization protocol |
Microarrays were hybridized and washed according to manufacturer's protocol (version 6.5).
|
| Scan protocol |
Scanned on an Agilent G2505C scanner
|
| Description |
Technical replicate 2
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1)
|
| |
|
| Submission date |
Sep 04, 2018 |
| Last update date |
Oct 07, 2020 |
| Contact name |
Eva González-Suárez |
| E-mail(s) |
[email protected]
|
| Organization name |
Institut d'Investigació Biomèdica de Bellvitge
|
| Department |
Epigenetics and Cancer Biology
|
| Lab |
Transformation and Metastasis
|
| Street address |
Hospital Duran i Reynals - 3a planta -Gran Via de l'Hospitalet, 199
|
| City |
Hospitalet de Llobregat |
| State/province |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE119464 |
CD45- sorted tumor cells from RANK+/+ and RANK-/- MMTV_PYMT late carcinomas |
|
