|
| Status |
Public on Mar 27, 2019 |
| Title |
HSD F0 female rep01 |
| Sample type |
RNA |
| |
|
| Source name |
whole fly, 14 days of treatment
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole fly
generation: F0
diet fed: HSD
diet of ancestral f0 males: --
strain/background: Oregon R
gender: female
age: 14 days
developmental stage: adult
batch: batch_01
|
| Treatment protocol |
At the end of treatment, flies were anesthetized using CO2 and collected in Trizol.
|
| Growth protocol |
30 adult virgin females (and unmated males) Oregon R flies per vial fed respective diet for 14 days at 25C temperature. Media vials changed after 7 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (RNeasy Microarray Tissue Handbook).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (GeneChip 3’ IVT Express Kit).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Drosophila_F0_Female_D01
Gene expression data using Affymetrix Drosophila Genome 2.0 array.
|
| Data processing |
The data was analysed using R/Bioconductor affy and limma packages. Quantile Normalization and RMA background correction was used.
|
| |
|
| Submission date |
Sep 13, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Manoj Ramesh Teltumbade |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Genomics and Integrative Biology
|
| Lab |
Drosophila Lab
|
| Street address |
Mathura Road, Near Sukhdev Vihar, bus depo
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110025 |
| Country |
India |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE119927 |
Transcriptomic profiling in a Drosophila model of diet induced transgenerational epigenetic inheritance. |
|
