|
| Status |
Public on Feb 01, 2009 |
| Title |
5 weeks 30 degrees rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
White adipose tissue from mice kept 5 weeks at room temperature
|
| Organism |
Mus musculus |
| Characteristics |
age: 6-8 weeks
gender: Male
strain: C57/BL6
|
| Treatment protocol |
Mice were adapted at 18˚C for 1-3 weeks before being transferred to 4˚C for 1 or 5 weeks.
|
| Growth protocol |
Male mice, 6-8-wk old, of the C57/Bl6 strain, which had been maintained at 22˚C were divided into groups.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix single round amplification protocol from 5 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
|
| Description |
Gene expression data from control mice
|
| Data processing |
GCRMA
|
| |
|
| Submission date |
Nov 01, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ola Larsson |
| E-mail(s) |
[email protected]
|
| Phone |
+46 (0)8 517 73280
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of oncology-pathology
|
| Street address |
CCK, R8:01
|
| City |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
|
| Relations |
| Reanalyzed by |
GSE119085 |
