|
| Status |
Public on Dec 31, 2018 |
| Title |
Female_Achl_RNAi_CT28 |
| Sample type |
SRA |
| |
|
| Source name |
heads
|
| Organism |
Drosophila melanogaster |
| Characteristics |
gender: Female
genotype: Achl_RNAi
time: CT28
tissue: heads
|
| Growth protocol |
3-5 day old adult flies were collected. Flies were maintained on standard food at 25 degrees under 12hr:12hr light:dark condition. The incubator light scheme was adjusted to constant darkness 24 hours before collection starts and maintained under constant darkness duringt the enitire collection period.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
fly heads were manually dissected in PBS and homogenized in Ttizol (Life Technologies), total RNAs were collected using Phase lock gels (5Prime) and RNeasy mini kit (Qiagen)
libraries were prepared with 200ng total RNA per sample using TruSeq Stranded mRNA LT prep kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
STAR was used to align raw reads to the reference Drosophila genome and transcriptome, RSEM was used to quantify the expression.
Genome_build: dm6
Supplementary_files_format_and_content: STAR-RSEM-generated TPM per gene per sample
|
| |
|
| Submission date |
Sep 18, 2018 |
| Last update date |
Dec 31, 2018 |
| Contact name |
Jiajia Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Washington University in St. Louis
|
| Department |
Department of Medicine
|
| Lab |
Hughes Lab
|
| Street address |
9952 CSRB, 4939 Children's Place.
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL21306 |
| Series (1) |
| GSE120100 |
Achilles-mediated and sex-specific regulation of circadian mRNA rhythms in Drosophila |
|
| Relations |
| BioSample |
SAMN10081342 |
| SRA |
SRX4707378 |
