|
| Status |
Public on Jan 05, 2009 |
| Title |
Carboplatin 24 hours set 2 |
| Sample type |
RNA |
| |
|
| Source name |
36M2 cell line from ovarian serous carcinoma cells
|
| Organism |
Homo sapiens |
| Characteristics |
Carboplatin
Carboplatin 24 hours set 2
|
| Treatment protocol |
we treated the 36M2 cell line with carboplatin 100μM or vehicle-control for 24hrs and cells were harvested and processed for RNA isolation at 24, 30 and 36hrs after treatment.
|
| Growth protocol |
Cells were grown in RPMI 1640 media with L-glutamine, 10% fetal bovine serum and 1% of penicillin/streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini Kit (Qiagen Valencia, CA, USA) according to manufacturer’s instructions
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis and hybridization on oligonucleotide microarrays (U133 Plus 2.0 Array GeneChip, Affymetrix, Inc., Santa Clara, CA) containing approximately 54,700 transcripts were carried out using standard protocols.
|
| |
|
| Hybridization protocol |
cDNA synthesis and hybridization on oligonucleotide microarrays (U133 Plus 2.0 Array GeneChip, Affymetrix, Inc., Santa Clara, CA) containing approximately 54,700 transcripts were carried out using standard protocols.
|
| Scan protocol |
The chips were scanned in an HIP chip scanner (Affymetrix)
|
| Description |
Gene expression data from 36M2 trated with Carboplatin for 24hrs
|
| Data processing |
Robust Multi-Array (RMA)
|
| |
|
| Submission date |
Nov 07, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
dimitrios Spentzos |
| E-mail(s) |
[email protected]
|
| Phone |
6176670586
|
| Organization name |
Beth ISrael Deaconess Medical center
|
| Department |
Department of medicine
|
| Street address |
330 Brookilne Ave CLS 541
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE13525 |
Carboplatin-induced gene expression changes in vitro are prognostic of survival in epithelial ovarian cancer |
|
| Relations |
| Reanalyzed by |
GSE119087 |
