|
| Status |
Public on Apr 01, 2009 |
| Title |
Biofilm_Outside_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Outside of biofilm in MOPS media with 0.2% glucose
|
| Organism |
Escherichia coli |
| Characteristics |
Strain: Escherichia coli MG1655 pOX38Km
|
| Growth protocol |
Samples were grown in MOPS minimal media with 0.2% glucose.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were RNA-stabilized with Qiagen RNAProtect Bacterial Reagent, and total RNA was isolated with Qiagen RNeasy mini kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
The isolated RNA was converted to cDNA, and the synthesized cDNA was fragmented and labeled with GeneChip DNA Labeling Reagent according to Affymetrix protocols.
|
| |
|
| Hybridization protocol |
Affymetrix protocols
|
| Scan protocol |
Affymetrix protocols
|
| Description |
Biofilm sample on glass surface
|
| Data processing |
Normalization was performed with dchip software using the Invariant Set Normalization method. Sample Planktonic_Stationary_rep2 was used as the baseline array.
|
| |
|
| Submission date |
Nov 13, 2008 |
| Last update date |
Nov 13, 2008 |
| Contact name |
Akinobu Ito |
| E-mail(s) |
[email protected]
|
| Phone |
+81-11-706-7588
|
| Fax |
+81-11-706-7588
|
| Organization name |
Hokkaido University
|
| Department |
Graduate School of Engineering
|
| Lab |
Water Quality Control Engineering
|
| Street address |
North-13, West-8, Kitaku
|
| City |
Sapporo |
| ZIP/Postal code |
060-8628 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3154 |
| Series (1) |
| GSE13589 |
Gene expression of E. coli MG1655 pOX38Km at the outside and inside of biofilms |
|
