disease state: Nasopharyngeal carcinoma stage: III, T2, N2, M0, G4
Extracted molecule
total RNA
Extraction protocol
RNA was extracted from biopsy samples using TRIzol Reagent (Invitrogen, USA) and purified by the RNeasy Mini Kit (Qiagen, UK).
Label
biotin
Label protocol
The cDNA was synthesized, in vitro transcribed and labeled using standard Affymetrix protocols.
Hybridization protocol
Affymetrix Standard protocol
Scan protocol
Affymetrix Standard protocol
Description
Stage III, T2, N2, M0, G4
Data processing
The scanned images of microarray chips were analyzed using the Affymetrix GenChip Operating Software (GCOS) with the default settings except that the target signal was set to 100.
