|
| Status |
Public on Feb 08, 2019 |
| Title |
AP2-G-DD +Shld1 78 hpi |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
AP2-G-DD +Shld1
|
| Organism |
Plasmodium falciparum |
| Characteristics |
strain: AP2-G-DD
hpi: 78
treatment: Shld1
|
| Treatment protocol |
0.5 μM Shld1 was added and maintained starting at 24 hpi to stabilize the AP2-G-DD protein. For the negative control, the same volume of ethanol was instead added.
|
| Growth protocol |
Parasites were cultured at 37°C in the presence of 6% oxygen and 5% carbon dioxide, in RPMI 1640 media supplemented with hypoxanthine, 0.5% Albumax II (Invitrogen), and 2.5 nM WR99210.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Starting at 30 hpi and every six hours up to 90 hpi, samples were harvested in Trizol for total RNA extraction.
|
| Label |
Cy5
|
| Label protocol |
Painter, H. J., Altenhofen, L. M., Kafsack, B. F. C. & Llinás, M. Whole-genome analysis of Plasmodium spp. Utilizing a new agilent technologies DNA microarray platform. Methods Mol. Biol. 923, 213–219 (2013).
|
| |
|
| Channel 2 |
| Source name |
3D7 Mixed Stage Reference Pool + 63% cDNA from samples
|
| Organism |
Plasmodium falciparum |
| Characteristics |
strain: 3D7 + AP2-G-DD
|
| Treatment protocol |
0.5 μM Shld1 was added and maintained starting at 24 hpi to stabilize the AP2-G-DD protein. For the negative control, the same volume of ethanol was instead added.
|
| Growth protocol |
Parasites were cultured at 37°C in the presence of 6% oxygen and 5% carbon dioxide, in RPMI 1640 media supplemented with hypoxanthine, 0.5% Albumax II (Invitrogen), and 2.5 nM WR99210.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Starting at 30 hpi and every six hours up to 90 hpi, samples were harvested in Trizol for total RNA extraction.
|
| Label |
Cy3
|
| Label protocol |
Painter, H. J., Altenhofen, L. M., Kafsack, B. F. C. & Llinás, M. Whole-genome analysis of Plasmodium spp. Utilizing a new agilent technologies DNA microarray platform. Methods Mol. Biol. 923, 213–219 (2013).
|
| |
|
| |
| Hybridization protocol |
Painter, H. J., Altenhofen, L. M., Kafsack, B. F. C. & Llinás, M. Whole-genome analysis of Plasmodium spp. Utilizing a new agilent technologies DNA microarray platform. Methods Mol. Biol. 923, 213–219 (2013).
|
| Scan protocol |
Agilent G2600D Microarray Scanner. Images were quantified using Agilent Feature Extraction Software (version 11.5.1.1).
|
| Description |
AP2-G-DD +Shld1 78 hpi
|
| Data processing |
Agilent Feature Extraction Software (v 11.5.1.1) was used for background subtraction.
|
| |
|
| Submission date |
Oct 09, 2018 |
| Last update date |
Feb 10, 2019 |
| Contact name |
Manuel Llinas |
| E-mail(s) |
[email protected]
|
| Phone |
8148673444
|
| Organization name |
Penn State University
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Manuel Llinas Lab
|
| Street address |
Millennium Science Complex, Pollock Rd
|
| City |
University Park |
| State/province |
Pennsylvania |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platform ID |
GPL15130 |
| Series (2) |
| GSE120990 |
PfAP2-G DD +Shld1 vs. -Shld1 expression timecourse |
| GSE125566 |
Regulation of sexual differentiation is linked to invasion in malaria parasites |
|
