|
| Status |
Public on Mar 30, 2019 |
| Title |
Cortex-rTg4510-Veh-4 |
| Sample type |
RNA |
| |
|
| Source name |
Brain cortex in rTg4510 mouse, Normal feed
|
| Organism |
Mus musculus |
| Characteristics |
strain: rTg4510
genotype/variation: Tg(Camk2a-tTA)1Mmay Tg(tetO-MAPT*P301L)4510Kha/J
Sex: F
treatment: none (normal feed)
tissue: brain cortex
|
| Treatment protocol |
Either normal feed or feed treated with Doxycycline (200ppm) for 35d
|
| Growth protocol |
Standard housing in a 14 hr light/10 hr dark cycle at a constant temperature (23°C±2°C) with food and water available ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction according to manufacturer's instructions from LN2 frozen brain samples.
|
| Label |
biotin
|
| Label protocol |
Commercially analyzed by ThermoFisher
|
| |
|
| Hybridization protocol |
Commercially analyzed by ThermoFisher
|
| Scan protocol |
Commercially analyzed by ThermoFisher
|
| Description |
rTg4510 Veh 4
Tg(Camk2a-tTA)1Mmay Tg(tetO-MAPT*P301L)4510Kha/J fed normal feed. Expresses human 0N4R P301L tau at approx 13x endongenous murine tau levels.
|
| Data processing |
Signal intensities were calculated using SST-RMA and are reported on the log 2 scale. Transcript clusters were annotated to gene symbols using NetAffx annotation files (Release 36).
|
| |
|
| Submission date |
Oct 15, 2018 |
| Last update date |
Mar 31, 2019 |
| Contact name |
Jose F Abisambra |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Florida
|
| Department |
Department of Neuroscience & Center for Translational Research in Neurodegenerative Research
|
| Lab |
Abisambra Lab
|
| Street address |
1275 Center Dr
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE121264 |
Tau drives translational selectivity by interacting with ribosomal proteins |
|
