|
| Status |
Public on Nov 22, 2008 |
| Title |
Muscle_Saline_01 |
| Sample type |
RNA |
| |
|
| Source name |
Saline
|
| Organism |
Mus musculus |
| Characteristics |
male C57BL/6
gastrocnemius muscle
|
| Treatment protocol |
Mice treated with intraperitoneal injections of JA16 anti-myostatin antibody or saline control on days 1 (0.12 mg/g body weight) and 3 (0.09 mg/g); mice were killed by CO2 inhalation on day5 and gatrocnemius muscle were immediately frozen in liquid nitrogen and stored at -70 degrees C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted by Polytron homogenization in Trizol reagent (Invitrogen), followed by RNA cleanup, precipitation, and recovery in water per instructions provided by Invitrogen
|
| Label |
biotin
|
| Label protocol |
RNA was reverse transcribed and cDNA was amplified, fragmented, and biotinylated with kits from NuGEN (version 2 Ovation and Version 2 Fragmention & Labeling) as recommended by NuGEN
|
| |
|
| Hybridization protocol |
3.75 micrograms fragmented, biotinylated cDNA was hybridized overnight in 220 ul volume at 45 degrees C in standard Affymetrix eukaryotic hybridization buffer; arrays were washed, stained with phycoerythrin-streptavidin (including anti-phycoerythrin antibody enhancement step) with Affymetrix Fluidics Station 450 as recommended by Affymetrix
|
| Scan protocol |
Affymetrix Scanner 3000 was used to generate DAT, CEL, and CHP files as recommended by Affymetrix
|
| Description |
139_Welle_M430_2.CEL
|
| Data processing |
The MAS5 algorithm was implemented with Affymetrix Gene Chip Operating Software
|
| |
|
| Submission date |
Nov 21, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Stephen Welle |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Rochester
|
| Street address |
601 Elmwood Avenue
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE13707 |
Effect of an anti-myostatin antibody on skeletal muscle gene expression in mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
