|
| Status |
Public on Mar 09, 2009 |
| Title |
Muscle Domestic 1 GF007022 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Muscle Wild pool
|
| Organism |
Oncorhynchus kisutch |
| Characteristics |
Wild RNA pool
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Reverse transcriptase of 10ug of total RNA was performed following the SuperScript II Reverse Transcriptase protocol (Invitrogen). Indirect labeling was performed on individual cDNA following the Array 50 kit protocol (Genisphere).
|
| |
|
| Channel 2 |
| Source name |
Muscle Domestic individual 1
|
| Organism |
Oncorhynchus kisutch |
| Characteristics |
Domestic RNA individual 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Reverse transcriptase of 10ug of total RNA was performed following the SuperScript II Reverse Transcriptase protocol (Invitrogen). Indirect labeling was performed on individual cDNA following the Array 50 kit protocol (Genisphere).
|
| |
|
| |
| Hybridization protocol |
Hybridization followed the procedures at http://web.uvic.ca/grasp/microarray (Genisphere Array 50 Protocol).
|
| Scan protocol |
Fluorescence images were collected at 10μm resolution using a ScanArray microarray scanner (Perkin Elmer). The same laser power (95%) and photomultiplier tube (PMT) settings were used for all slides (Cy3 PMT 75, Cy5 PMT 70). Fluorescence intensity data were extracted from TIF images using QuantArray version 3.0 software (Perkin Elmer).
|
| Description |
none
|
| Data processing |
Data transformation (setting background corrected values <0.01 to 0.01 and Lowess normalization) and analysis were performed in GeneSpring GX 7.3.1 software (Agilent Technologies). Spots with intensity less than the empty spot mean plus 2.5 times their standard deviation were excluded from the analysis.
|
| |
|
| Submission date |
Dec 05, 2008 |
| Last update date |
Mar 09, 2009 |
| Contact name |
Dionne Sakhrani |
| E-mail(s) |
[email protected]
|
| Organization name |
Fisheries & Oceans Canada
|
| Street address |
4160 Marine Drive
|
| City |
West Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V7V 1N6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL2716 |
| Series (1) |
| GSE13846 |
Domestication and growth hormone transgenesis cause similar changes in gene expression profiles in coho salmon |
|
