|
| Status |
Public on Dec 08, 2008 |
| Title |
Larval Feeding Mid Gut rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Larval Feeding Mid Gut
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Canton-S, 30 feeding third instar larvae
|
| Treatment protocol |
Flies were anaesthetised briefly by chilling on ice, then immediately dissected.
|
| Growth protocol |
Wild-type Drosophila melanogaster (Canton S strain) were raised on standard medium on a 12:12 h L:D cycle, at 23C, and at 55% r.h. To facilitate the collection of accurately staged adults, a laying population of around 12 males and 12 females were transferred to fresh vials daily. When adults subsequently emerged, they were transferred to fresh vials on the day of emergence, and used 7 days later. Where larvae were used, these were third instar.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Micro kit (Invitrogen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA (or according to the affy IVT protocol) were hybridized for 16 hr at 45C on GeneChip Drosophila Genome 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip scanner 3000 7G.
|
| Description |
The midgut, extending from behind the proventriculus to just in front of the insertion of the Malpighian tubules, dissected from third-instar feeding larvae.
amplification: 1-round
|
| Data processing |
The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 08, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jing Wang or Venkat Chintapalli |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
0141 3306212
|
| Fax |
3304878
|
| Organization name |
University of Glasgow
|
| Department |
Molecular Genetics
|
| Lab |
Dow/Davis lab
|
| Street address |
Dumbarton Road
|
| City |
Glasgow |
| State/province |
Scotland |
| ZIP/Postal code |
G11 6NU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE7763 |
Using FlyAtlas to identify better Drosophila models of human disease |
|
| Relations |
| Reanalyzed by |
GSE119084 |
