|
| Status |
Public on Nov 23, 2018 |
| Title |
7312A-1 |
| Sample type |
RNA |
| |
|
| Source name |
HEK293T cells transfected with Vpx 7312A expression vector
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293T
cell type: fibroblast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by mirVanaTM RNA Isolation Kit (Applied Biosystem p/n AM1556 ) following the manufacturer's instructions.
|
| Label |
cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 μg RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
not provided
|
| Scan protocol |
not provided
|
| Description |
Gene expression data from HEK293T cells transfected with Vpx 7312A expression vector
|
| Data processing |
Samples were analyzed with Affymetrix software.
|
| |
|
| Submission date |
Nov 23, 2018 |
| Last update date |
Nov 26, 2018 |
| Contact name |
Hanchu Xiong |
| E-mail(s) |
[email protected]
|
| Organization name |
Cancer Institute, Second Affiliated Hospital, College of Medicine, Zhejiang University
|
| Street address |
Jiefang Road NO.88
|
| City |
Hangzhou |
| ZIP/Postal code |
310000 |
| Country |
China |
| |
|
| Platform ID |
GPL23178 |
| Series (1) |
|
