|
| Status |
Public on Apr 23, 2019 |
| Title |
WT TH1 cells, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse CXCR5-SLAMhighCD4+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
tissue: spleen
cell type: CD4 T cells
cell subtype: TH1
genotype: WT
|
| Treatment protocol |
Splenocytes were isolated and antigen-specific CD4+ T cells were enriched using Biotin-labeled anti-CD19, anti-Ter119, anti-CD8, anti-CD11c, anti-CD11b, anti-NK1.1, anti-CD25 and magnetic bead purification (Beaver). Virus specific SMtg CD4+CD45.1+CD19-L/D- cells were then sorted into TFH and TH1 populations on the basis of CXCR5 and SLAM expression using a FACS Aria II (BD Bioscience).
|
| Growth protocol |
all infection were done by intraperitoneal injection of 2 x 10^5 plaque-forming units (PFU) of LCMV Armstrong strain per mouse. 2 x 10^5 naïve SMARTA CD4+T cells were adoptively transferred to recipient mice prior to LCMV infection. Spleens were harvested 6 days after LCMV infection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sorted cells were lysed with TRIzol LS reagent (Life Technologies). Total RNA was extracted with isopropyl ethanol.
|
| Label |
biotin
|
| Label protocol |
RNA quality of all samples was confirmed by BioAnalyzer Nano gel (Agilent), then probes were generated from 30ng total RNA by single round linear amplification using the Ovation Pico system (Nugen).
|
| |
|
| Hybridization protocol |
Standard Affymetrix
|
| Scan protocol |
Standard Affymetrix
|
| Description |
Gene exprssion data from TH1 cells. (CXCR5-SLAMhighCD4+ T cells from LCMV infected mice)
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Nov 26, 2018 |
| Last update date |
Apr 23, 2019 |
| Contact name |
Pengcheng Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
Army Medical University
|
| Department |
Institute of Immunology
|
| Street address |
gaotanyan street
|
| City |
Chongqing |
| State/province |
chongqing |
| ZIP/Postal code |
400038 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE122931 |
Expression data from WT and Tbx21-/- in vivo TFH cells versus TH1 cells |
|
