|
| Status |
Public on Dec 30, 2008 |
| Title |
hrp36-1.b KD |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Non-specific RNAi control
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Schneider SL2 cells
|
| Treatment protocol |
10 µg of dsRNA at day 0 and repeated at day 2
|
| Growth protocol |
2x10e6 S2 cells exponentially growing in Schneider's media were seeded in 6 well plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
At day 4, total RNA is extracted using the RNAeasy mini kit and the QiaShredder (Qiagen) following the manufacturor protocol.
|
| Label |
Cy3
|
| Label protocol |
A custom labeling protocol described in Blanchette et al. (2005) Genes Dev., PMID: 15937219
|
| |
|
| Channel 2 |
| Source name |
treated with hrp36 dsRNA for 4 days
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Schneider SL2 cells
|
| Treatment protocol |
10 µg of dsRNA at day 0 and repeated at day 2
|
| Growth protocol |
2x10e6 S2 cells exponentially growing in Schneider's media were seeded in 6 well plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
At day 4, total RNA is extracted using the RNAeasy mini kit and the QiaShredder (Qiagen) following the manufacturor protocol.
|
| Label |
Cy5
|
| Label protocol |
A custom labeling protocol described in Blanchette et al. (2005) Genes Dev., PMID: 15937219
|
| |
|
| |
| Hybridization protocol |
A custom protocol, see Blanchette et al. (2005) Genes Dev, PMID: 15937219 as well as Castle et al. (2003) Genome Biology, PMID: 14519201
|
| Scan protocol |
Feature Extractor version 7.5.1. Scanner settings can be found in the raw data file.
|
| Description |
Hrp36 knock-down from dsRNA B sample 1
|
| Data processing |
Detail description of the data processing can be found in Blanchette et al. (2005). Genes and Dev. PMID: 15937219
|
| |
|
| Submission date |
Dec 14, 2008 |
| Last update date |
Dec 29, 2008 |
| Contact name |
Marco Blanchette |
| E-mail(s) |
[email protected]
|
| Phone |
415-272-4837
|
| Organization name |
Stowers Institute for Medical Research
|
| Lab |
Marco Blanchette
|
| Street address |
1000 E. 50th St.
|
| City |
Kansas City |
| State/province |
MO |
| ZIP/Postal code |
64110 |
| Country |
USA |
| |
|
| Platform ID |
GPL7508 |
| Series (2) |
| GSE13940 |
Genome-wide analysis of alternative pre-mRNA splicing of the Drosophila hnRNP A/B family members |
| GSE13944 |
Splicing_Arrays_2 |
|
