|
| Status |
Public on Dec 02, 2019 |
| Title |
D. Melanogaster_DGE_zt3_25C |
| Sample type |
SRA |
| |
|
| Source name |
fly heads
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Canton-S
circadian timepoints: zt3
temperature: 25C
tissue: fly heads
|
| Treatment protocol |
RNA extraction was done using TRI Reagent [SIGMA] following manufacturer´s protocol.
|
| Growth protocol |
Flies were grown at different temperatures
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
The precipitated RNAwas taken for library preparation.
3’-seq libraries were prepared as previously described (Shishki et al., Afik et al., 2017)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
temperature_GTTTATGC_13.1_25C.zt3
|
| Data processing |
Ribosomal RNA was taken out using Bowtie2 aligner against the ribosomal RNA
Reads were aligned to dm3 genome using TopHat
The aligned reads were analyzed with cufflinks
Genome_build: dm3
Supplementary_files_format_and_content: table of transcripts reads
|
| |
|
| Submission date |
Dec 19, 2018 |
| Last update date |
Dec 02, 2019 |
| Contact name |
Sebastian Kadener |
| E-mail(s) |
[email protected]
|
| Organization name |
Brandeis University
|
| Street address |
415 South Street
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
9190401 |
| Country |
USA |
| |
|
| Platform ID |
GPL19132 |
| Series (2) |
| GSE124136 |
Differences in circadian rhythms in fly at different temperatures |
| GSE124141 |
RNA seq from fly heads at different temperatures and circadian timepoints [independent replicate 2] |
|
| Relations |
| BioSample |
SAMN10616493 |
| SRA |
SRX5167199 |
