|
| Status |
Public on Dec 02, 2019 |
| Title |
Drosophila heads at 25C rep1 RNA immunoprecipitation AGO1-IP |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila heads RNA at 25C IP of AGO1-Ip
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Canton-S
age: adult flies
temperature: 25C
tissue: heads
|
| Treatment protocol |
Flies were grown at either 18, 25 or 19 degrees. Heads were collected and AGO1-IP was eprformed as in Kadener et al. 2009
|
| Growth protocol |
FLies were grown at 18C, 25C, 29C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was then used to prepare a probe that was hybridized to Drosophila 2.0 gene expression arrays (Affymetrix) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
IP_25.1.
Drosophila_T1-1_IP
|
| Data processing |
CEL files were imported to Affymetrix Expression Console program, and data were normalized using Robust Multichips Analysis. Differential expression was tested for probes that had expression levels above 4.0 (log2 scale) in at least one group (mutants or WT). All other probes were excluded from the analysis.
|
| |
|
| Submission date |
Dec 20, 2018 |
| Last update date |
Dec 02, 2019 |
| Contact name |
Sebastian Kadener |
| E-mail(s) |
[email protected]
|
| Organization name |
Brandeis University
|
| Street address |
415 South Street
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
9190401 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE124136 |
Differences in circadian rhythms in fly at different temperatures |
| GSE124201 |
Expression data from Drosophila heads at difeerent temperatues, after AGO1 immnuprecipitation or input [microarray] |
|
