|
| Status |
Public on Jan 01, 2022 |
| Title |
w1118, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Embryos of w1118 flies at 0 – 24 hr were collected
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain background: w1118
genotype/variation: wild type
tissue: embryos at 0 - 24 hr
|
| Growth protocol |
All flies were maintained at 25 °C on standard corn meal unless specified.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Lipid Tissue Mini Kit, Qiagen
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 400.
|
| Scan protocol |
Gene chips were scaned using Affymetrix GeneChip Scanner 3000.
|
| Data processing |
Gene expression data was preprocessed using rma (R package affy)
|
| |
|
| Submission date |
Jan 14, 2019 |
| Last update date |
Jan 01, 2022 |
| Contact name |
Ying Ge |
| E-mail(s) |
[email protected]
|
| Organization name |
Dalian Medical University
|
| Street address |
9 Western Section, Lvshun South Street
|
| City |
Dalian |
| ZIP/Postal code |
116044 |
| Country |
China |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE125032 |
Caliban regulates mitochondrial morphology and redox state in enterocytes to maintain intestinal homeostasis in Drosophila |
|
