|
| Status |
Public on Nov 11, 2010 |
| Title |
Pollen tube growth, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Stigma and Ovary 15-25 min after pollination
|
| Organism |
Oryza sativa |
| Characteristics |
cv. Nipponbare
|
| Treatment protocol |
Tissue samples were collected on August - October. Collected biological samples were frozen in liquid nitrogen and kept -80C until RNA extraction.
|
| Growth protocol |
Plants were grown in paddy fields under normal condition at Mishima, Japan.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy plant mini kit (Qiagen) according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 500 ng total RNA using One-Cycle cDNA Synthesis Kit (Affymetrix) according to manufacturers protocol (hws_kit_manual).
|
| |
|
| Hybridization protocol |
3.3 ug of labeled and fragmented cRNA was hybridized to GeneChip Rice Genome Array using GeneChip Hybridization Oven 640 (Affymetrix). Wash and stain was performed by GeneChip Fluidics Station 450 (Affymetrix). All operations were performed with Hybridization, Stain and Wash Kit (Affymetrix) according to manufacturer's protocol (hws_kit_manual).
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data from pollinated stigma and ovary. Pollinated pollen grains were included in collected samples.
|
| Data processing |
CEL files produced by GCOS 1.3 were analyzed using the statistical software R with bioconductor package “affy”. Signal intensities were extracted by expresso algorithm with parameters : bgcorrect.method = “mas”, normalize = “F”, pmcorrect.method = “pmonly”, summary.method = “mas”. Extracted signal intensities were introduced into GeneSpring 7.3.1 (Agilent) and scaled to 75th percentile per chip. Mas5calls algorithm in package “affy” was used for detection call.
|
| |
|
| Submission date |
Jan 06, 2009 |
| Last update date |
Nov 11, 2010 |
| Contact name |
Nori Kurata |
| E-mail(s) |
[email protected]
|
| Phone |
+81-55-981-6808
|
| Organization name |
National Institute of Genetics
|
| Lab |
Plant genetics Lab.
|
| Street address |
1111 Yata
|
| City |
Mishima |
| State/province |
Shizuoka |
| ZIP/Postal code |
411-8540 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2025 |
| Series (2) |
| GSE14298 |
Rice expression atlas (2): Pollination - Fertilization |
| GSE14304 |
Rice expression atlas: Plant reproductive process |
|
