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Sample GSM359245 Query DataSets for GSM359245
Status Public on Mar 15, 2009
Title Aireko_1
Sample type RNA
 
Source name Aire-deficient mTEC
Organism Mus musculus
Characteristics Strain: C57BL/6
Treatment protocol none
Extracted molecule total RNA
Extraction protocol RNA extraction was performed using the RNeasy Micro Kit (Qiagen) according to manufacturer's instructions.
Label biotin
Label protocol 100ng of total RNA was amplified using T7-oligo dT and the Megascript T7 kit (Ambion). A second round of cDNA synthesis was performed using first round amplified RNA.
Amplified RNA was biotin-labelled using the GeneChip IVT Labelling Kit.
 
Hybridization protocol 15 micro-grams of labelled RNA were then fragmented as described in the Affymetrix manual. The mouse genome 430 2.0 arrays were hybridised overnight and washed.
Scan protocol Microarrays were scanned using a GeneChip Scanner 3000 (Affymetrix).
Description Gene expression data from Aire-deficient mTECs, first biological replicate
Data processing The intensities for each probe set were normalized and summarized using the Robust Multi-array Analysis (RMA) algorithm in the software programme R.
 
Submission date Jan 09, 2009
Last update date Aug 28, 2018
Contact name Francois-Xavier Hubert
E-mail(s) [email protected]
Organization name The Walter and Eliza Hall Institute of Medical Research
Department Immunology
Street address 1G Royal Parade
City Melbourne
State/province Victoria
ZIP/Postal code 3052
Country Australia
 
Platform ID GPL1261
Series (1)
GSE14365 Expression data from WT and Aire deficient mTECs
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Normalized intensities as outputted by the RMA algorithm.

Data table
ID_REF VALUE
1415670_at 7.608275115
1415671_at 8.48483766
1415672_at 8.928802601
1415673_at 3.313460548
1415674_a_at 6.536197388
1415675_at 4.874536244
1415676_a_at 9.449844368
1415677_at 5.600265628
1415678_at 8.688729159
1415679_at 8.724063633
1415680_at 6.398163461
1415681_at 4.782707335
1415682_at 4.418490527
1415683_at 10.04999755
1415684_at 3.752859488
1415685_at 6.320736154
1415686_at 8.748582519
1415687_a_at 11.00855763
1415688_at 7.351943021
1415689_s_at 6.127580073

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM359245.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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