|
Status |
Public on Mar 15, 2009 |
Title |
Aireko_1 |
Sample type |
RNA |
|
|
Source name |
Aire-deficient mTEC
|
Organism |
Mus musculus |
Characteristics |
Strain: C57BL/6
|
Treatment protocol |
none
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extraction was performed using the RNeasy Micro Kit (Qiagen) according to manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
100ng of total RNA was amplified using T7-oligo dT and the Megascript T7 kit (Ambion). A second round of cDNA synthesis was performed using first round amplified RNA. Amplified RNA was biotin-labelled using the GeneChip IVT Labelling Kit.
|
|
|
Hybridization protocol |
15 micro-grams of labelled RNA were then fragmented as described in the Affymetrix manual. The mouse genome 430 2.0 arrays were hybridised overnight and washed.
|
Scan protocol |
Microarrays were scanned using a GeneChip Scanner 3000 (Affymetrix).
|
Description |
Gene expression data from Aire-deficient mTECs, first biological replicate
|
Data processing |
The intensities for each probe set were normalized and summarized using the Robust Multi-array Analysis (RMA) algorithm in the software programme R.
|
|
|
Submission date |
Jan 09, 2009 |
Last update date |
Aug 28, 2018 |
Contact name |
Francois-Xavier Hubert |
E-mail(s) |
[email protected]
|
Organization name |
The Walter and Eliza Hall Institute of Medical Research
|
Department |
Immunology
|
Street address |
1G Royal Parade
|
City |
Melbourne |
State/province |
Victoria |
ZIP/Postal code |
3052 |
Country |
Australia |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE14365 |
Expression data from WT and Aire deficient mTECs |
|
Relations |
Reanalyzed by |
GSE119085 |