|
| Status |
Public on Mar 15, 2009 |
| Title |
Aireko_2 |
| Sample type |
RNA |
| |
|
| Source name |
Aire-deficient mTEC
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
|
| Treatment protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using the RNeasy Micro Kit (Qiagen) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA was amplified using T7-oligo dT and the Megascript T7 kit (Ambion). A second round of cDNA synthesis was performed using first round amplified RNA.
Amplified RNA was biotin-labelled using the GeneChip IVT Labelling Kit.
|
| |
|
| Hybridization protocol |
15 micro-grams of labelled RNA were then fragmented as described in the Affymetrix manual. The mouse genome 430 2.0 arrays were hybridised overnight and washed.
|
| Scan protocol |
Microarrays were scanned using a GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from Aire-deficient mTECs, second biological replicate
|
| Data processing |
The intensities for each probe set were normalized and summarized using the Robust Multi-array Analysis (RMA) algorithm in the software programme R.
|
| |
|
| Submission date |
Jan 09, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Francois-Xavier Hubert |
| E-mail(s) |
[email protected]
|
| Organization name |
The Walter and Eliza Hall Institute of Medical Research
|
| Department |
Immunology
|
| Street address |
1G Royal Parade
|
| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE14365 |
Expression data from WT and Aire deficient mTECs |
|
| Relations |
| Reanalyzed by |
GSE119085 |
