|
| Status |
Public on Dec 10, 2019 |
| Title |
DT.80 |
| Sample type |
SRA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Liver cells
strain line: 1912
Sex: F
obesity: Yes
diabetes: No
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation was performed using commercial kit of QIAGEN (Cat.No.73404), and Quality control of the RNA samples was tested by 2100 BioAnalyzer (Agilent). Illumina TruSeq Stranded RNA Sample Prep Kit was used.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Sequence alignment was performed with Tophat2
Cufflinks was used to quantify expression
Cuffdiff was used to compare the level of gene expression
Genome_build: GRCm.38
|
| |
|
| Submission date |
Feb 13, 2019 |
| Last update date |
Dec 10, 2019 |
| Contact name |
Aristotelis Chatziioannou |
| E-mail(s) |
[email protected]
|
| Organization name |
National Hellenic Research Foundation
|
| Street address |
48 Vassileos Constantinou Avenue
|
| City |
Athens |
| ZIP/Postal code |
11635 |
| Country |
Greece |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE126490 |
Uncovering the hepatic gene expression variations and sex-differences as response to high-fat diet consumption in the highly genetically diversity of the Collaborative Cross mouse model |
|
| Relations |
| BioSample |
SAMN10924377 |
| SRA |
SRX5371655 |
