|
| Status |
Public on Feb 22, 2022 |
| Title |
A2308_3 |
| Sample type |
SRA |
| |
|
| Source name |
B. abortus A2308
|
| Organism |
Brucella abortus 2308 |
| Characteristics |
strain: 2308
genotype: control
|
| Treatment protocol |
B. abortus 2308 was obatined from IVDC, and the clpP mutant was constructed by homologous recombination
|
| Growth protocol |
B. abortus 2308 and the clpP mutant were grown in TSB at 37℃ until the log phase was reached
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately, The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
Gram-negative bacteria
|
| Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
HISAT2.1.0 mapping to reference genome
count counting
Genome_build: GCA_000054005
Supplementary_files_format_and_content: TXT file contain counts for each sample
|
| |
|
| Submission date |
Feb 22, 2019 |
| Last update date |
Feb 22, 2022 |
| Contact name |
Hao Dong |
| E-mail(s) |
[email protected]
|
| Phone |
+86-10-53852705
|
| Organization name |
National Institutes for Food and Drug Control
|
| Street address |
Huatuo road NO.31
|
| City |
Beijing |
| ZIP/Postal code |
102618 |
| Country |
China |
| |
|
| Platform ID |
GPL26214 |
| Series (1) |
| GSE126915 |
Transcriptome Analysis of the different expressed genes affected by ClpP in B. abortus 2308 |
|
| Relations |
| BioSample |
SAMN10986397 |
| SRA |
SRX5406977 |
