Three replicates, each containing 10 adult female flies, were collected and frozen in -70 ̊ C prior to RNA extraction
Growth protocol
Flies were cultivated and crossed in vials with potato-yeast-agar medium at 25 ̊ C.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated using TRIzol reagent (Invitrogen) followed by a purification using RNeasy kit (Qiagen) according to the instruction by the suppliers.
Label
biotin
Label protocol
Standard Affymetrix
Hybridization protocol
Standard Affymetrix
Scan protocol
Standard Affymetrix
Description
Gene expression data from adult females JH_h4-2
Data processing
Raw data was normalized using RMA (R and Bioconductor).
