|
| Status |
Public on Oct 26, 2010 |
| Title |
Leaf L6 of miR156 over-expressed, biological repeat 2 |
| Sample type |
RNA |
| |
|
| Source name |
Rice overexpressed miR156, biological repeat 2
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
mir156 expression: over-expressing
line: Md43
|
| Growth protocol |
Sterilized seeds of transgenic plants and wild type were germinated in MS medium. Positive transgenic plants were selected by adding 50 mM hygromycin for miR156-overexpressed plants. Ten-day-old uniform seedlings were transferred to hydroponics culture medium and forty five-day-old plants were collected for microarray analysis. The leaves in the main tiller were numbered according to its emerging order, with L1 indicating the first developed leaf. All samples were prechecked by small RNA gel blot for the expression level of miR156 to confirm overexpression. The old (L3) and young leaf (L6) were used for microarray hybridization.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol(Invitrogen, Carlsbad, CA, USA) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
GeneChips were scanned using the Affymetrix® GeneChip® Scanner 3000.
|
| Data processing |
Affymetrix ® GeneChip® Operating SoftwareVersion1.0
|
| |
|
| Submission date |
Feb 03, 2009 |
| Last update date |
Sep 06, 2011 |
| Contact name |
Kabin Xie |
| E-mail(s) |
[email protected]
|
| Phone |
+86-27-87281716
|
| Fax |
+86-27-87297092
|
| URL |
http://www.ncpgr.cn/
|
| Organization name |
Huazhong Agriculture University
|
| Department |
National Key Laboratory of Crop Genetic Improvement
|
| Lab |
National Center of Plant Gene Research (Wuhan)
|
| Street address |
Shizi Shan Street
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE14692 |
Temporal regulatory role of miR156 during leaf development |
|
