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Sample GSM366998 Query DataSets for GSM366998
Status Public on Mar 01, 2010
Title hsfb1 hsfb2b double knockout vs wild type #3
Sample type RNA
 
Channel 1
Source name wild-type seedling
Organism Arabidopsis thaliana
Characteristics tissue: seedling
age: 7 days
strain: Col-0
genotype: wild-type
growth condition: normal
Biomaterial provider Miho Ikeda
Treatment protocol No treatment
Growth protocol Grown under long day (16hr light), 22 degree temprature in MS agar medium. Grown together with mutant plants in same dish.
Extracted molecule total RNA
Extraction protocol By using RNeasy plant mini kit supplied by QIAGEN.
Label Cy3
Label protocol By using Quick Amp Labeling Kit supplied by Agilent.
 
Channel 2
Source name hsfb1 hsfb2b seedling
Organism Arabidopsis thaliana
Characteristics tissue: seedling
age: 7 days
strain: Col-0
genotype: hsfb1 hsfb2b
mutation description: hsfb1 hsfb2b double knockout
growth condition: normal
Biomaterial provider Miho Ikeda
Treatment protocol No treatment
Growth protocol Grown under long day (16hr light), 22 degree temprature in MS agar medium. Grown together with control plants in same dish.
Extracted molecule total RNA
Extraction protocol By using RNeasy plant mini kit supplied by QIAGEN.
Label Cy5
Label protocol By using Quick Amp Labeling Kit supplied by Agilent.
 
 
Hybridization protocol As described in manual supplied by Agilent.
Scan protocol As described in manual supplied by Agilent.
Description Biological replicate 3 of 4.
Data processing By using Feature Extraction version 9.1 supplied by Agilent.
 
Submission date Feb 04, 2009
Last update date Oct 26, 2009
Contact name Nobutaka Mitsuda
E-mail(s) [email protected]
Organization name National Institute of Advanced Industrial Science and Technology
Department Bioproduction Research Institute
Lab Gene Regulation Research Group
Street address Central 6 Higashi 1-1-1
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8566
Country Japan
 
Platform ID GPL6177
Series (2)
GSE14702 Functional analysis of HSFB transcription factors [22°C]
GSE31888 Functional analysis of Arabidopsis HSFB transcription factor

Data table header descriptions
ID_REF
VALUE Log10 target/control signal
gProcessedSignal Background-subtracted signal value of Cy3 (control)
rProcessedSignal Background-subtracted signal value of Cy5 (target)
gIsFeatNonUnifOL Boolean value representing whether the spot's Cy3 signal is non-uniformaty outlier
rIsFeatNonUnifOL Boolean value representing whether the spot's Cy5 signal is non-uniformaty outlier
gIsFeatPopnOL Boolean value representing whether the spot's Cy3 signal is population outlier
rIsFeatPopnOL Boolean value representing whether the spot's Cy5 signal is population outlier
gIsPosAndSignif Boolean value representing whether the spot's Cy3 signal exceeds significantly background
rIsPosAndSignif Boolean value representing whether the spot's Cy5 signal exceeds significantly background
gIsWellAboveBG Boolean value representing whether the spot's Cy3 signal exceeds more significantly background
rIsWellAboveBG Boolean value representing whether the spot's Cy5 signal exceeds more significantly background

Data table
ID_REF VALUE gProcessedSignal rProcessedSignal gIsFeatNonUnifOL rIsFeatNonUnifOL gIsFeatPopnOL rIsFeatPopnOL gIsPosAndSignif rIsPosAndSignif gIsWellAboveBG rIsWellAboveBG
1 1.86E-02 2.84E+02 2.96E+02 0 0 0 0 1 1 1 1
2 0.00E+00 3.94E+00 3.49E+00 0 0 0 0 0 0 0 0
3 0.00E+00 3.96E+00 3.51E+00 0 0 0 0 0 0 0 0
4 0.00E+00 3.97E+00 3.53E+00 0 0 0 0 0 0 0 0
5 0.00E+00 3.99E+00 3.55E+00 0 0 0 0 0 0 0 0
6 0.00E+00 4.00E+00 3.57E+00 0 0 0 0 0 0 0 0
7 0.00E+00 4.01E+00 3.59E+00 0 0 0 0 0 0 0 0
8 0.00E+00 4.03E+00 3.61E+00 0 0 0 0 0 0 0 0
9 0.00E+00 4.04E+00 3.62E+00 0 0 0 0 0 0 0 0
10 0.00E+00 4.05E+00 3.64E+00 0 0 0 0 0 0 0 0
11 0.00E+00 4.06E+00 3.66E+00 0 0 0 0 0 0 0 0
12 0.00E+00 4.07E+00 3.67E+00 0 0 0 0 0 0 0 0
13 -2.95E-01 3.21E+01 1.63E+01 0 0 0 0 1 1 1 0
14 0.00E+00 4.10E+00 3.71E+00 0 0 0 0 0 0 0 0
15 1.93E-01 1.52E+03 2.37E+03 0 0 0 0 1 1 1 1
16 0.00E+00 4.12E+00 3.74E+00 0 0 0 0 0 0 0 0
17 -2.55E-01 1.51E+01 8.37E+00 0 0 0 0 1 1 0 0
18 4.41E-02 9.88E+02 1.09E+03 0 0 0 0 1 1 1 1
19 -4.17E-02 1.41E+03 1.28E+03 0 0 0 0 1 1 1 1
20 7.18E-02 1.61E+02 1.90E+02 0 0 0 0 1 1 1 1

Total number of rows: 39102

Table truncated, full table size 1876 Kbytes.




Supplementary file Size Download File type/resource
GSM366998.txt.gz 3.5 Mb (ftp)(http) TXT
Processed data included within Sample table
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