At necropsy, samples of 16 tissues were collected, snap frozen in liquid nitrogen and stored at -80 ºC, until de RNA extraction.
Growth protocol
Pigs with one month of age were housed simultaneously, fed the same diets during the fattening period, that lasted two months.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted from 100 mg tissue using the RiboPure™ kit (Ambion, Austin, USA) according to the manufacturer’s protocol.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol
The hybridization was done according to Affymetrix standard protocols.
Scan protocol
Standard Affymetrix protocol
Description
Hypothalamus,Youli,male
Data processing
Microarray expression data were generated with GeneChip Operating Software (GCOS)
