|
| Status |
Public on Feb 10, 2009 |
| Title |
Su(var)3-9 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
D.m. adult heads Su(var)3-9
|
| Organism |
Drosophila melanogaster |
| Characteristics |
developmental stage: adult
tissue: heads
genotype: mutation in Su(var)3-9
|
| Treatment protocol |
N/A
|
| Growth protocol |
Flies were raised at 25C with 70% humidity on standard corn meal food.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol (Invitrogen) according to the manufacturer's recommendations.
|
| Label |
biotin
|
| Label protocol |
Preparation of the samples for hybridization was carried out following the protocols recommended by Affymetrix (see 3' IVT manual available at www.affymetrix.com).
|
| |
|
| Hybridization protocol |
Hybridization was carried out according to the protocols recommended by Affymetrix (see 3' IVT manual available at www.affymetrix.com) on a SS450 fluidics station.
|
| Scan protocol |
Scanning was carried out according to the protocols recommended by Affymetrix (see 3' IVT manual available at www.affymetrix.com) with a 7G3000 scanner.
|
| Description |
gene expression data from adult fly heads carrying a mutation in Su(var)3-9
|
| Data processing |
Data analysis was carried out using the Bioconductor packages affy and limma in R on the log2-transformed signal intensities. The RMA method was employed to carry out background correction, quantile normalization, and summarization of gene expression levels.
|
| |
|
| Submission date |
Feb 09, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nicole Christina Riddle |
| Organization name |
Washington University
|
| Department |
Biology
|
| Lab |
SCR Elgin
|
| Street address |
1 Brookings Dr.; CB1137
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63130 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE14756 |
Expression data from Drosophila histone methyltransferase mutants |
|
| Relations |
| Reanalyzed by |
GSE119084 |
