|
| Status |
Public on Apr 10, 2020 |
| Title |
ECA109 LV-GFP3 (S143) |
| Sample type |
SRA |
| |
|
| Source name |
esophageal squamous cell carcinoma cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: ECA109
genotype: NC
cell line source: esophageal squamous cell carcinoma (ESCC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately, The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
HISAT2.1.0 mapping to reference genome
count counting
Genome_build: GRCh38
Supplementary_files_format_and_content: TXT file contains counts for each sample
|
| |
|
| Submission date |
Mar 27, 2019 |
| Last update date |
Apr 10, 2020 |
| Contact name |
Yue Zhao |
| E-mail(s) |
[email protected]
|
| Phone |
+8618621279206
|
| Organization name |
Changhai Hospital
|
| Department |
Department of Thoracic Surgery
|
| Street address |
168 Changhai Road, Shanghai 200433, China
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE128914 |
RNA-Seq of LEF1 overexpression ECA109 cells |
|
| Relations |
| BioSample |
SAMN11265385 |
| SRA |
SRX5581240 |
