|
| Status |
Public on Oct 01, 2009 |
| Title |
B-ALL, No molecular abnormalities 4 (microRNA) |
| Sample type |
RNA |
| |
|
| Source name |
Bone Marrow
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age: 42
tissue: Bone Marrow
phenotype: pre B
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by isopropanol precipitation.
|
| Label |
biotin
|
| Label protocol |
Lc Sciences proprietary labeling method which utilizes an affinity tag for signal amplification after miRNA hybridization to the chip
|
| |
|
| Hybridization protocol |
Hybridization was performed overnight on a µParaflo microfluidic chip using a micro-circulation pump (Atactic Technologies)
|
| Scan protocol |
Axon GenePix 4000B Microarray Scanner
|
| Description |
Gene expression data from patient with newly diagnosed ALL (percentage of leukemic cells at least 80%)
|
| Data processing |
Data were analyzed with R. Signal values from each array were background subtracted (array background value was set as quantified by the service provider), quantile-normalized between the 7 intra-array replicates and the signal value for each miRNA summarized as the average of repeating spots. Processed miRNA intensity values were normalized between-array by quantile normalization.
|
| |
|
| Submission date |
Feb 13, 2009 |
| Last update date |
Aug 24, 2009 |
| Contact name |
Teresa Colombo |
| Organization name |
Consiglio Nazionale delle Ricerche (CNR)
|
| Department |
Institute of Molecular Biology and Pathology (IBPM)
|
| Street address |
p.le A. Moro 7
|
| City |
Rome |
| ZIP/Postal code |
00185 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8191 |
| Series (1) |
| GSE14834 |
Characterization of B- and T-lineage ALL by Integrated Analysis of microRNA and mRNA Expression Profiles |
|
