|
| Status |
Public on Nov 01, 2009 |
| Title |
3T3-L1 adipocyte tissue culture, CLA+metformin fed, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
3T3-L1 adipocyte tissue culture, CLA+metformin
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 3T3-L1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
|
| Description |
n/a
|
| Data processing |
The data was analysed using R 2.7.2 with Bioconductor 2.2. Arrays were normalised via gcRMA, and all treatments were compared to the control LA via the limma package. Bonferroni-Hochberg adjusted p-values were used as significance measurement.
|
| |
|
| Submission date |
Feb 18, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jean-Jack M. Riethoven |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Nebraska-Lincoln
|
| Department |
Beadle Center for Biotechnology
|
| Lab |
Bioinformatics Core Research Facility
|
| Street address |
1901 Vine Street
|
| City |
Lincoln |
| State/province |
NE |
| ZIP/Postal code |
68588-0665 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE14888 |
Conjugated linoleic acid activates AMPK and reduces adiposity more effectively when used with metformin |
|
| Relations |
| Reanalyzed by |
GSE119085 |
