|
| Status |
Public on Feb 21, 2009 |
| Title |
Female_CAST14D_D24 |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis, 14 days
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
condition: cast
time: 14 days
|
| Growth protocol |
Recreationally active, non-smoking, healthy men (N=12) and women (N=12) participated in the trial. Demographics included: age (men = 20.5 ± 1.7; women = 21.1 ± 2.7 y), height (men = 178.4 ± 5.4; women = 164.3 ± 5.5 cm), and body mass index (men = 24.3 ± 3.5; women = 22.8 ± 2.2) (Table 1). All of the women were eumenorrheic and ~ 50 % were tested in each phase of the menstrual cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from muscle using a commercially available TRIzol® Reagent (Invitrogen Canada) following the manufacturer’s instructions. The concentration and purity of the RNA was spectrophotometrically determined by measuring the absorbance at 260 (OD260) and 280 (OD280) with an OD260/OD280 ratio of ~1.8 indicative of good quality RNA. The integrity of RNA samples were assessed by RNA agarose-formaldehyde gel electrophoresis and evaluating the ratio of 28S to 18S rRNA bands.
|
| Label |
biotin
|
| Label protocol |
Standard Affy labeling protocol.
|
| |
|
| Hybridization protocol |
Standard Affy hybridization protocol.
|
| Scan protocol |
Standard Affy scan protocol.
|
| Description |
MU_CAST14D_D24_E06_Signal
|
| Data processing |
Global changes in gene expression were examined using the HG U133 plus gene micro-array (Affymetrix, Santa Clara, CA) which contains 54,675 human probe sequences. The background correction, normalization and derivation of expression measure were based on the Affymetrix signal (MAS 5.0 algorithm).
|
| |
|
| Submission date |
Feb 20, 2009 |
| Last update date |
Feb 20, 2009 |
| Contact name |
Simon Melov |
| Organization name |
Buck Institute for Research on Aging
|
| Lab |
Melov Lab
|
| Street address |
8001 Redwood Blvd
|
| City |
Novato |
| State/province |
CA |
| ZIP/Postal code |
94945 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14901 |
Limb immobilization induces a coordinate down-regulation of mitochondrial and other metabolic pathways in men and women |
|
