|
| Status |
Public on Mar 02, 2020 |
| Title |
Fn3sCas12a - NT gRNA [Fn3-NT_S4] |
| Sample type |
SRA |
| |
|
| Source name |
uncleaved DNA from the randomized protospacer-adjacent motif (PAM) library
|
| Organism |
synthetic construct |
| Characteristics |
in vitro environment: myTXTL from Arbor Biosciences
lysate origination: E. coli
molecule subtype: DNA from PCR-amplification
|
| Treatment protocol |
Prior to library construction, the TXTL reaction should be diluted 1:10 for successful amplification using a high-fidelity polymerase.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
myTXTL extract purchased from Arbor Biosciences.
The samples were PCR amplified twice. The 1st PCR involved attachment of unique adapters that provided a landing site for the 2nd PCR. The 2nd PCR involved attachment of i5 and i7 Nextera low plex pooling indices.
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
Fn3-NT_S4_L001_R1
|
| Data processing |
see the following website: https://bitbucket.org/csmaxwell/crispr-txtl-pam-counting-script/overview
Real Time Analysis for basecalling
Supplementary_files_format_and_content: .csv files
|
| |
|
| Submission date |
Apr 26, 2019 |
| Last update date |
Mar 02, 2020 |
| Contact name |
Chase Beisel |
| E-mail(s) |
[email protected]
|
| Organization name |
North Carolina State University
|
| Department |
Chemical and Biomolecular Engineering
|
| Lab |
Partners II, Rm 2110
|
| Street address |
840 Main Campus Drive
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27606 |
| Country |
USA |
| |
|
| Platform ID |
GPL17769 |
| Series (1) |
| GSE130377 |
Randomized PAM depletion of phylogenetically-diverse Cas12a nucleases |
|
| Relations |
| BioSample |
SAMN11515446 |
| SRA |
SRX5758289 |
