|
| Status |
Public on Apr 30, 2009 |
| Title |
Expressional Response to Acute Hypoxia in Drosophila (NC1) |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila melanogaster: control group 1
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Canton-S
developmental stage: Adult
tissue: Whole organism
|
| Treatment protocol |
25 males and females (5-6 days old adult) flies exposed to 2.5 hours of normoxia kept in the same room so exposed to same level of light and noise
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Micro kit (Invitrogen) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip scanner 3000 7G
|
| Description |
Control Sample 1
|
| Data processing |
The data were analyzed with GCOS1.4 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 24, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Dan Zhou |
| E-mail(s) |
[email protected]
|
| Phone |
858-822-6889
|
| Fax |
858-534-6971
|
| Organization name |
University of California, San Diego, School of Medicine
|
| Department |
Pediatrics
|
| Lab |
Haddad Lab
|
| Street address |
CMG 103, 9500 Gilman Drive, MC0735
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0735 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE14981 |
Distinct Mechanisms Underlying Tolerance to Intermittent and Constant Hypoxia in Drosophila melanogaster |
|
| Relations |
| Reanalyzed by |
GSE119084 |
