|
| Status |
Public on May 02, 2020 |
| Title |
H3K27ac_KIT_WT_rep1 [4299] |
| Sample type |
SRA |
| |
|
| Source name |
H3K27ac_KIT_WT
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: wild type
age: P7
tissue: testis
cell type: c-Kit+ spermatogonia
chip antibody: H3K27ac
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Germ cells were isolated from mouse testis by STA-PUT method or MACS. Cells were fixed in 1% formaldehyde for 10 min at RT. Nuclei were isolated by lysis buffer. Chromatin was sonicated by Covaris sonicater and used for ChIP experiments.
see ChIPmentation (PMID: 26280331)
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
4299
pC51D06F-A408-D5B6-EAD8-EDE9308D7E27
|
| Data processing |
Data analysis was performed using the BioWardrobe (PMID: 26248465 )
Briefly, reads were mapped using Bowtie and peaks were identified using MACS2
Genome_build: mm10
Supplementary_files_format_and_content: csv file includes identified peaks for each sample
|
| |
|
| Submission date |
May 02, 2019 |
| Last update date |
May 03, 2020 |
| Contact name |
Satoshi Namekawa |
| E-mail(s) |
[email protected]
|
| Phone |
513-803-1377
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE130652 |
Active enhancer landscape during spermatogenesis. |
|
| Relations |
| BioSample |
SAMN11567427 |
| SRA |
SRX5783561 |
