|
| Status |
Public on May 07, 2019 |
| Title |
RNA-seq_ILC3a_1 |
| Sample type |
SRA |
| |
|
| Source name |
tonsil
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tonsil
batch: 1
celltype: ILC3a
markers: CD56+ NKp44+ CD196+ CD300LF+ CD103-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Briefly, tissue was mechanically disrupted, passed through 0.0059 inches opening sieve, and filtered extensively through 70μm cell strainers. CD56+ cells were enriched via positive selection with CD56 microbeads (Myltenyi), according to manufacturer’s recommendations. RNAs from the sorted ILC subsets were prepared by using RNeasy Plus Micro kit (Qiagen), following manufacturer instructions.
cDNA was amplified with SMARTer Universal Low Input RNA Kit (Clontech). RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
gene_counts.txt
sample name in processed data file:
sample.1
|
| Data processing |
RNA seq counts were determined using STAR
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited files include raw counts for each sample
|
| |
|
| Submission date |
May 06, 2019 |
| Last update date |
May 07, 2019 |
| Contact name |
Marco Colonna |
| E-mail(s) |
[email protected]
|
| Organization name |
Washington University in St. Louis
|
| Street address |
660 S. Euclid Ave.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE130772 |
RNA-Seq from human ILC transitional populations |
| GSE130775 |
Expression profiling of ILC transitional populations and Aiolos accessability and H3K27ac histone modifications in transfected MNK3 cells |
|
| Relations |
| BioSample |
SAMN11582880 |
| SRA |
SRX5797717 |
