|
| Status |
Public on Oct 29, 2019 |
| Title |
pPCL_N12 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow plasma cells
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Primary plasma cell leukemia (pPCL)
gender: Male
del13q: No
gain1q: Yes
del1p: Yes
t(11;14): Yes
t(4;14): No
t(14;16): No
status of the other p53 allele: Wild type
|
| Treatment protocol |
Not applicable
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA and DNA were extracted using AllPrep DNA/RNA Mini Kit (Qiagen). RNA integrity was assessed using Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer's instructions (Affymetrix)
|
| |
|
| Hybridization protocol |
Hybridization was performed on Affymetrix GeneChip HTA 2.0 Array as per the manufacturers protocol.
|
| Scan protocol |
Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450 and GeneChip Scanner 7G).
|
| Data processing |
Raw data were quantile normalized, background corrected and log2 transformed with the Robust Multi-array Average (RMA) algorithm using the oligo package (v.1.44.0) in R (v.3.5.0). BrainArray custom CDF files (version 19 Ensembl) at gene level were used as reference.
hta20hsensgcdf_19.0.0.cdf
|
| |
|
| Submission date |
May 14, 2019 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Norma C. Gutiérrez |
| E-mail(s) |
[email protected]
|
| Phone |
+34923291384
|
| Organization name |
Centro de Investigación del Cáncer de Salamanca
|
| Department |
Hematologia
|
| Lab |
12
|
| Street address |
Campus Miguel de Unamuno
|
| City |
Salamanca |
| State/province |
Salamanca |
| ZIP/Postal code |
37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL20932 |
| Series (1) |
| GSE131216 |
Transcriptome analysis reveals significant differences between primary plasma cell leukemia and multiple myeloma even when sharing a similar genetic background |
|
