|
| Status |
Public on Mar 13, 2009 |
| Title |
865 |
| Sample type |
RNA |
| |
|
| Source name |
Frozen DLBCL sample
|
| Organism |
Homo sapiens |
| Characteristics |
tumor site: extra-nodal
cell of origin defined by ihc: Non-GCB
infiltrating t-cells: 1+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 21 frozen primary DLBCL samples using Trizol Method.
|
| Label |
Cy3
|
| Label protocol |
Perfomed following manufacturer's guidelines
|
| |
|
| Hybridization protocol |
100ng of Cy3-labelled cRNA were hybridized to microarray slides (8 complete microarrays/slide) and washed following manufacturer’s guidelines.
|
| Scan protocol |
The arrays were scanned and the intensity of the hybridization signals obtained using the Feature Extraction software (Agilent Technologies).
|
| Description |
miRNA expression from frozen DLBCL sample
|
| Data processing |
The gene-level signal values of the miRNAs were extracted from individual text files generated by the Agilent software (we used the “gTotalGeneSignal” column”). 470 human miRNAs were used in the downstream analysis. To adjust for hybridization differences between arrays, normalization was performed to scale all the signal values in one array by a multiplicative factor, so that the 75th percentile of the signal values in each array is the same value.
|
| |
|
| Submission date |
Mar 06, 2009 |
| Last update date |
Jan 29, 2010 |
| Contact name |
Cheng Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Biostatistics and Computational Biology
|
| Street address |
44 Binney St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL9081 |
| Series (2) |
| GSE15142 |
Copy number abnormality, MYC activity and the genetic fingerprint of normal B-cells and the microRNA profile of DLBCL-21 |
| GSE15225 |
microRNA profile of DLBCL |
|
